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Microscope Tips

Cleaning Optics
Microscope lens are made of coated, soft glass and should be treated with great care because they can be easily
scratched. To clean your lens never use a dissecting tool or other hard instrument. To clean the objective tips and
lens, use an aspirator or soft camel's hair brush to remove any unwanted dirt. Then moisten the end of a Q-tip™
with a lens cleaner or lens paper and clean the optical surface using a circular motion. Use the other end of the
dry Q-tip™
or lens paper to dry the optical lens, then use the aspirator to remove any loose particles.

Adjusting the Interpupillary Distance
If you have a binocular microscope, you will need to adjust the interpupillary distance, which is the distance between
the center of your two pupils. Each person has his or her own interpupillary distance therefore each microscope must
be adjusted for each persons distance.  If you look through the eyepieces and see two images, the interpupillary
distance is not correct. Focus on the specimen and slide the eyepieces closer together or farther apart until the
two fields merge to form a single circle.

Adjusting the Diopter
The diopter adjust is used to compensate for the differences between your eyes. Each eyepiece is equipped with a
diopter adjustment ring by which can be adjusted independently of the other eyepiece. Each user should adjust
the diopter prior to using the microscope to receive optimal quality and comfort during viewing.

1. Adjust the interpupillary distance until it is correct for you.
2. With a test slide in position on the stage, rotate the 10X ocular lens into position.
3. Rotate the diopter adjustment ring (= tube length adjustment ring) of the left eyepiece until its numerical value is the
   same as your
interpupillary distance.
4. Close your right eye and bring an object into clear sharp focus using the coarse and fine adjustment when necessary.
5. Close your left eye and bring the same object into focus using the diopter ring only on the right eyepiece.

Note: Do not use the focus knob for the last step--only the diopter adjustment.

How To Use the Immersion Objective (100x Oil)

Be sure to use only immersion oil for microscopy. Other types of oil may damage the surface of the lens.

1. Focus on the specimen by switching the objectives to the lowest to the highest magnification.

2. Before using  the 100x immersion objective, place a drop of immersion oil provided with the 100x
objective onto the specimen where you will be observing. The small drop of oil must be directly over
the area of the specimen being examined.

3. Rotate the nosepiece to the 100x immersion objective, so that the objective touches the oil,then focus
using the fine adjustment knob. Air bubbles in the oil will affect the image quality, so make sure that the
oil is free of bubbles. To remove bubbles, turn the revolving nosepiece to move the oil immersion objective
back and forth a few times.

4. When finished, remove the oil from the front lens of the objective by wiping it with Xylene, Kimwipes
or a non soluble optics cleaner.  Ensure to remove any excess dust that may build up using an

Using the right coverslip thickness with your microscope is essential to prevent any aberrations or lose of contrast
of your image. Below is a chart of the standard coverslips used. Walter Products offers #1 glass coverslips 0.13-0.17
thickness, which are designed for most microscope objectives.

 Coverslip No.
Coverslip Size (mm)
  #0   0.08-0.12mm
  #1   0.13-0.17mm
  #2   0.16-0.19mm
  #2.5   0.17-.25mm

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